Finally,
the PCR You Truly Need.
Beyond complexity. Beyond compromise. Welcome to the future of multiplex PCR development, powered by Mila™ AI. Let us make the Best Kit Possible for you.
Discover Mila
Good PCR is No longer Enough.
The complexity of multiplex PCR has always forced compromises:
- Too few targets, or too many irrelevant ones
- Generic assays that miss your organisms of interest
- Kits that favor simplicity over performance
- Long lead times or rigid catalog options
Why settle for “close enough” when your reality demands precision?
With Mila™, we can create PCR kits tailored from the ground up using artificial intelligence, with the exact targets, coverage, and balance you need.
No guesswork. No compromises. Just the right kit, made possible
Introducing Mila
Let us Design the Impossible.
Mila™ is the world’s first AI-powered platform that liberates multiplex PCR development:
- Too few targets, or too many irrelevant ones
- Generic assays that miss your organisms of interest
- Kits that favor simplicity over performance
- Long lead times or rigid catalog options
With Mila™, we can create PCR kits tailored from the ground up using artificial intelligence, with the exact targets, coverage, and balance you need.
No guesswork. No compromises. Just the right kit, made possible
What Makes Mila™ Unique
The BEST kit, evading THE REAL COMPLEXITIES of Multiplex PCR.
Freedom
Flexibility
Partnership
Who We Serve
We Walk with the Visionaries.
Mila™ is trusted by industry pioneers and leaders who refuse to settle. Together, we have created PCR kits that define new standards in food safety, diagnostics, and biotechnology.
Products: Crafted by Mila™, Perfected as Ampliora™
When vision meets intelligence, you get Ampliora™, a line of the best tailor-made PCR kits that redefine what’s possible.
Download Ampliora CatalogProducts we have developed with our clients.
| Ampliora™ 2.3 Listeria spp. and L. monocytogenes SPID | |
| Ampliora™ 2.8 Listeria spp. and Salmonella spp. SPID | |
| Ampliora™ 3.5 Salmonella spp. L. monocytogenes and Listeria spp. SPID |
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| Ampliora™ F39 E. coli STEC, E. coli O157:H7and Salmonella spp. SPID | |
| Ampliora™ 8.1 Beer Yeast Plus SPID | |
| Ampliora™ 8.2 Beer Bacteria Plus SPID | |
| Ampliora™ 4.7 Spoilage Beverage SPID | |
| Ampliora™ 6.1 WaterScan Plus SPID |
Why it works
Behind Mila™ lies deep learning, system modeling, and advanced combinatorial logic, all designed to eliminate guesswork and maximize assay performance.
There are more ways to build a 4-target PCR test, than stars in the milky way.
A typical qPCR assay requires three oligonucleotides (“oligos”): one forward primer, one reverse primer, and one hydrolysis probe for each target. Each of these oligos must be selected from many possibilities. Furthermore, the best primer/probe set for one target may no longer be the best option once a second target is added, since the second set of oligos can interact with the first. In a modest 4-target qPCR reaction, depicted in the figure above, there may be more possible primer/probe combinations than there are stars in the milky way galaxy. Finding the best combination by trial-and-error is nearly impossible.
How it works
Mila is a comprehensive platform for in silico PCR design. It retrieves and aligns all published sequences for the targets of interest, distinguishing sequencing errors from true variants and removing them. BLAST algorithms are used to select primers that effectively exclude potential off-target hybridizations while ensuring inclusivity. The remaining primer/probe sets undergo extensive thermodynamic modelling and AI-based optimization to select the set with the highest efficiency and lowest probability of undesirable interactions. This is accomplished in a computationally efficient manner, without testing every possibility. The algorithm considers a comprehensive range of variables, including the thermodynamics of nucleic acids, differences between fluorophores, melting and annealing temperatures, secondary structures, and the chemical properties of the reaction (pH, salt concentrations, other reagents, etc.)
